Proteomic Analysis of Plasma-Purified VLDL, LDL, and HDL Fractions from Atherosclerotic Patients Undergoing Carotid Endarterectomy: Identification of Serum Amyloid A as a Potential Marker

Figure 4

One-dimensional western blotting analysis. Bar charts reporting AP SAA and apo AI expression in VLDL, LDL, and HDL fractions isolated from 4 pooled plasma samples obtained from 57 controls (lanes 1–4) and 4 from 79 patients (lanes 5–8). Following data normalization for apo AI levels, AP SAA was confirmed as differentially expressed in all purified fractions from patients compared to controls (𝑃=0.03, 𝑃=0.03, and 𝑃=0.05, for VLDL, LDL, and HDL fractions, resp.). Results obtained on a randomly chosen sample from controls group (lane 9) and from patients group (lane 10) are shown. 10 μg and 1 μg of proteins were loaded for AP SAA and apo AI immunodetection, respectively.