Clinical Study
Protein Sulfhydryl Group Oxidation and Mixed-Disulfide Modifications in Stable and Unstable Human Carotid Plaques
Table 1
Differentially oxidised proteins identified after 2D electrophoresis by peptide mass fingerprinting analysis.
| | Spot no. | Identified protein | Accession no. (NCBI) | Theo. Mr (KDa) | Theo. pI | Matched peptides | Coverage (%) |
| | 1 | Transferrin | gi | 79.31 | 6.9 | 17 | 25 | | 2 | Human serum albumin | gi | 66.44 | 5.7 | 14 | 21 | | 3, 4 | Alpha-2HS-glycoprotein | gi | 40.11 | 5.4 | 3 | 23 | | 5, 6 | Alpha-1-antitrypsin | gi | 46.89 | 5.4 | 11 | 22 | | 7, 8, 9 | Fibrin beta | gi | 51.37 | 8.3 | 7 | 21 | | 10 | Vimentin | gi | 53.75 | 5.0 | 12 | 32 | | 11 | Vimentin | gi | 41.66 | 4.8 | 12 | 42 | | 12 | Vimentin | gi | 35.09 | 4.7 | 10 | 42 | | 13 | Alpha actin | gi | 42.34 | 5.2 | 10 | 36 | | 14 | Tropomyosin 1 | gi | 33.03 | 4.6 | 8 | 21 | | 15 | SP40 (Apo-J) | gi | 37.00 | 5.7 | 7 | 33 | | 16 | Modulator of apoptosis 1 (MAP-1) | gi | 39.72 | 5.2 | 3 | 14 | | 17, 18 | Tropomyosin 4 | gi | 28.62 | 4.7 | 7 | 26 | | 19, 20 | Heat shock 27 KDa protein 1 | gi | 22.82 | 6.0 | 8 | 44 |
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