Sulforaphane Enhances the Ability of Human Retinal Pigment Epithelial Cell against Oxidative Stress, and Its Effect on Gene Expression Profile Evaluated by Microarray Analysis
Figure 3
SF induces and increase in the transcripts for NQO1, SRXN1, and GCLM in the RPE 19 cells over time. qRT-PCR was used to show progressive increases over time in the amount of mRNA for NQO1 ((a), (b)), SRXN1 ((c), (d)), and GCLM ((e), (f)) following addition of SF to the cultures. GADPH was used as a loading control. The data for qRT-PCR were normalized with GADPH. The fold changes were calculated from the groups of 6 and 12 hours of SF treatment versus the 0 hour SF treatment. Data are presented as mean ± SD ( in each group). Significant differences are indicated by asterisks, ** represents .
