Sulforaphane Enhances the Ability of Human Retinal Pigment Epithelial Cell against Oxidative Stress, and Its Effect on Gene Expression Profile Evaluated by Microarray Analysis

Figure 4

SF increases expression of Trx1 in RPE19 cells. The amount of Trx1 mRNA was shown by qRT-PCR and sqRT-PCR ((a) and (b)) to increase at least 6 hours and remained higher than control levels at 12 hours. However, Western blot data ((c) and (d)) showed a progressive increase in the amount of Trx1 protein from 6 h to 12 h time points. Data are presented as mean ± SD (𝑛=5 in each group of real-time qRT-PCR; 𝑛=3 in each group of immunoblots). Significant differences are indicated by asterisk; * represents 𝑃<0.05, and ** represents 𝑃<0.01.

(a)

(b)

(c)

(d)