Sulforaphane Enhances the Ability of Human Retinal Pigment Epithelial Cell against Oxidative Stress, and Its Effect on Gene Expression Profile Evaluated by Microarray Analysis

Figure 6

Histogram presentation of fold change of Nrf2 quantified by real-time qRT-PCR (a) and images of sqRT-PCR with GADPH as loading control (b). Nuclear extractions of the cultured RPE 19 cells treated with or without SF were probed with anti-Nrf2 (c). Densitometric analysis of western blots (d). The amount of Nrf2 in the nuclear fraction increased in the presence of SF compared to untreated cells. Data are presented as mean ± SD (𝑛=5 in each group of real-time qRT-PCR; 𝑛=3 in each group of immunoblots). Significant differences are indicated by asterisk; * represents 𝑃<0.05, and ** represents 𝑃<0.01.

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