Enhanced 4-Hydroxynonenal Resistance in KEAP1 Silenced Human Colon Cancer Cells
Figure 2
Effect of KEAP1 knockdown on the expression of NRF2 target genes. (a) The basal mRNA levels for AKRs (AKR1C1/2, 1C2, 1C3, 1B1, and 1B10) were determined by real-time PCR analysis for relative quantification in the sc and shKEAP1 HT29 cells. (b) The basal mRNA levels for GCLC, GCLM, GSR, GSTA2, GSTA3, and GSTM2 were determined in the sc and shKEAP1 HT29 cells. (c) The basal mRNA levels for MRP2 and MRP3 were monitored in the sc and shKEAP1 HT29 cells. The values are relative levels with respect to the sc control group and are the means ± SD of 3-4 experiments. compared with the sc control.
