Enhanced 4-Hydroxynonenal Resistance in KEAP1 Silenced Human Colon Cancer Cells

Figure 6

Effects of AKR1C on H₂O₂-mediated cytotoxicity. (a) The levels of 4HNE adducts were measured in the sc and shKEAP1 HT29 cells following the incubation with H₂O₂ (200 μM) for 0–12 h. The bar graph represents relative intensities of 4HNE adducts/β-tubulin. Average intensities of two marked bands were measured and normalized with each β-tubulin intensity. (b) The sc and shKEAP1 HT29 cells were coincubated with flufenamic acid (F, 20 μM) and H₂O₂ (80 μM) for 24 h and cell viabilities were assessed using MTT analysis. The values are relative levels with respect to each vehicle group and are the means ± SD of 8 wells. ^a𝑃<0.05 compared with the sc control cell line. ^b𝑃<0.05 compared H₂O₂ alone treated scHT29 cells.

(a)

(b)