Saccharomyces cerevisiae Linker Histone—Hho1p Maintains Chromatin Loop Organization during Ageing
Figure 1
The lack of S. cerevisiae linker histone leads to slower growth rate during CLS and increased survival. (a) Yeast wild type and hho1delta cells were cultured in SD media for a period of 20 days. At certain time points aliquots were taken for spectrophotometric OD600 measurements and for counting the number of cells in a Nuebauer counting chamber. Results are presented as combined graphs comparing at one time the growth rate (lines) and the absolute number of cells at each time point (bars). Every time point stands for three independent repetitions of the experiments with STDVs (±). The difference between the wild type and hho1delta culture growth were statistically significant (). (b) The survival rate of hho1delta mutant cells and their progenitor wild type strains was assessed by plating 102 cells on YPD plates at every two days during the CLS 30°C for two days allowing cells to divide. CFUs were then counted and percentage survival was calculated by assuming the number of CFUs for each strain at 3rd day for 100% (lines). Additionally, the number of colonies was included in the graph (bars) thus allowing easy and comprehensible comparison between the CLS of wild type and hho1delta cells. was statistically insignificant only for CFUs at 5th day. At all other time points the differences between the wild type and hho1delta cultures were significant and for the simplicity of the graph presentation are not marked on the figure.
