Sweet Chestnut (Castanea sativa Mill.) Bark Extract: Cardiovascular Activity and Myocyte Protection against Oxidative Damage

Figure 3

Effect of CSM bark extract on intracellular ROS production in cardiomyocytes. Cardiomyocytes were treated with chestnut extract (1–100 μg/mL) for 24 h, oxidative damage was then induced exposing the cells to 100 μM H₂O₂ for 30 min, and intracellular ROS were determined using the peroxide-sensitive fluorescent probe DCFH-DA as described in Materials and Methods section. Data are expressed as percent of control cells treated with H₂O₂. Values represent means ± S.D. (𝑛=4). *𝑃<0.05 with respect to H₂O₂-treated cells.