Dietary Flavonoids as Therapeutics for Preterm Birth: Luteolin and Kaempferol Suppress Inflammation in Human Gestational Tissues In Vitro
Figure 4
Effect of luteolin and kaempferol treatment on NF-κB DNA binding activity and c-Jun expression. ((a), (c)) Primary myometrial cells were pretreated with 20 μM luteolin or 100 μM kaempferol for 24 h, followed by 30 min incubation with 500 pg/mL IL-1β. (a) Representative IκB-α Western blot from one patient using β-actin as a loading control. Similar data were obtained for another two patients. (b) Primary myometrial cells were pretreated with 20 μM luteolin or 100 μM kaempferol for 6 h, followed by treatment with 500 pg/mL IL-1β for 24 h. NF-κB p65 DNA binding activity was assayed using a NF-κB p65 transcription factor assay ( patients). Data is displayed as absorbance at 450 nm. Each bar represents the mean ± SEM. versus IL-1β-induced NF-κB DNA binding (one-way ANOVA). (c) Representative Western blot of nuclear c-Jun from one patient using Ponceau S as a loading control. Similar data were obtained for another four patients.
