Mitochondrial DNA Instability in Cells Lacking Aconitase Correlates with Iron Citrate Toxicity
Figure 3
Mutations in genes encoding citrate synthase prevent mtDNA loss due to aco1Δ. (a) Cells grown in YPD medium to mid-logarithmic phase were stained with DAPI and examined using fluorescence microscopy. WT (rho+), BY4741; aco1Δ, ZLY2630; cit1Δ aco1Δ, RBY469; cit2Δ aco1Δ, RBY277; cit1Δ cit2Δ aco1Δ, ZLY854; cit1Δ cit2Δ cit3Δ aco1Δ, ZLY854; rtg2Δ aco1Δ, ZLY2545. (b) Quantitative analysis of the percentage of rho0 cells in yeast strains based on DAPI-staining images.