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Oxidative Medicine and Cellular Longevity
Volume 2013, Article ID 517045, 9 pages
Research Article

The Effect of Alpha-Lipoic Acid on Mitochondrial Superoxide and Glucocorticoid-Induced Hypertension

1Department of Renal Medicine, St George Hospital, Kogarah, NSW 2217, Australia
2Department of Medicine, University of New South Wales, Sydney, NSW 2052, Australia
3John Curtin School of Medical Research, Australian National University, Canberra, ACT 0200, Australia
4Cardiovascular Research Unit, The Canberra Hospital, Canberra, ACT 2605, Australia

Received 11 July 2012; Accepted 4 January 2013

Academic Editor: Jose Magalhaes

Copyright © 2013 Sharon L. H. Ong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

This supplementary material provides additional information on some of the validation experiments performed in this study. The minimum MitoSOX concentration required to provide an optimal fluorescence signal in rat kidney cells was determined in the first validation study. MitoSOX concentration of 2 μM was identified as the lowest optimal concentration. MitoSOX geometric mean fluorescence intensity was increased in rat kidney cells pre-treated in Antimycin A (5 μM, positive control) and decreased in cells pre-treated with Tiron (50 mM, negative control).

  1. Supplementary Materials