Neurotoxic Effects of trans-Glutaconic Acid in Rats

Figure 3

In vitro effect of the antioxidants GSH (100 μM), melatonin (100 μM), L-NAME (500 μM), trolox (5 μM), and catalase (Cat) plus superoxide dismutase (SOD; 10 mU·mL⁻¹ each) on trans-glutaconic- (tGA-) induced alterations on thiobarbituric-acid reactive substances (TBA-RS) and glutathione (GSH) levels in cerebral cortex of 30-day-old rats. Cortical homogenates were preincubated for 15 min with the antioxidants before the addition of the 1 mM tGA. The experiments were performed in triplicate, are plotted as mean ± S.E.M. (𝑛=4-5), and are expressed as nmol·mg protein⁻¹. ^∗𝑃<0.05 and ^∗∗𝑃<0.01 compared to control group; ^#𝑃<0.05 compared to tGA group (Duncan multiple range test).