A Regulatory Role of NAD Redox Status on Flavin Cofactor Homeostasis in S. cerevisiae Mitochondria
Figure 8
NAD+ and NADH inhibition of FAD pyrophosphatase. The rate of FAD hydrolysis () was fluorimetrically measured in the standard medium at pH 7.5 with FAD at the indicated concentrations (2.5, 5, 7.5, or 12.5 μM) and solubilized SCM (0.05 mg protein). NAD+ or NADH was added in the FAD degradation reaction mixture at the concentration indicated. When NADH sensitivity was tested, KCN (1 mM) was added to the reaction mixture. The Dixon plots of the inhibition by NAD+ and NADH are reported in panels (a) and (b), respectively.
