Maintenance of Mitochondrial Morphology by Autophagy and Its Role in High Glucose Effects on Chronological Lifespan of Saccharomyces cerevisiae
Figure 6
ROS levels in S. cerevisiae at 24 h, 48 h, and 72 h of growth. Wild-type cells grown in 0.5%, 2%, and 4% glucose were collected at the indicated times and stained with 5 μg/mL DHE to detect superoxide radicals. Fluorescence intensities were analyzed by flow cytometry. (a) The clear and filled histograms represent the cells without and with DHE, respectively. (b) Percentage cells showing elevated ROS levels at each incubation time for cells grown in 0.5% glucose (clear rectangles), 2% glucose (lighter grey rectangles), and 4% glucose (darker grey rectangles). Data are the averages from two independent experiments; bars indicate the range of data obtained.
