Maintenance of Mitochondrial Morphology by Autophagy and Its Role in High Glucose Effects on Chronological Lifespan of Saccharomyces cerevisiae

Figure 7

The shortened chronological lifespan of S. cerevisiae cells grown in higher concentrations of glucose can be reversed without genetic manipulation. Wild-type cells were grown in media containing 0.5%, 2%, and 4% glucose for 48 hours and exchanged into the conditioned media originally supplemented with 0.5% (0.5% 48 h) or 4% (4% 48 h) glucose. Cell viability was assessed by spotting diluted cultures onto YPD plates at indicated times and compared with that of the cells without any media exchange (no change).