Enhanced Prostacyclin Synthesis by Adenoviral Gene Transfer Reduced Glial Activation and Ameliorated Dopaminergic Dysfunction in Hemiparkinsonian Rats
Figure 4
Infective tropism of adenovirus encoding GFP (AdGFP) in mesencephalic neuron/glial cultures and GFP expression in AdGFP-infected mixed glial cells. (a)(e) are representative micrographs of double labeling staining of cultures maintained in serum-free condition; Green color: GFP-immunoreactivity (IR). Red color in (a) III tubulin-IR for neurons, (b) tyrosine hydroxylase (TH)-IR for dopamine neurons, (c) GFAP-IR for astroglia, (d) ED1-IR for microglia, (e) NG2-IR, Magnification 200, (f) mixed glial cells, magnification 100. Arrows or arrow head in the figure indicates double-staining IR. AdGFP (106 pfu/well each) was added to cultured cells in serum or serum-free medium. The infective tropism of AdGFP in serum-free condition was % GFAP(+) cells, % ED1(+) cells, and % NG2(+) cells. GFP(+) cells in neuron/glial cultures in serum-free and serum-containing conditions during infection were and cells/mm2, respectively.
