Atherogenesis May Involve the Prooxidant and Proinflammatory Effects of Ferryl Hemoglobin
Figure 4
Oxidized LDL and atheroma lipids cause Hb oxidation and formation of ferrylHb. (a) Human Hb (20 μmol/L heme) was treated with H2O2 (200 μmol/L), native LDL (400 μg/mL), oxLDL (400 μg/mL), and lipid derived from human atherosclerostic plaque (LP) (400 μg/mL). After 90 minutes of incubation Hb samples (4 μg/lane) were subjected to SDS-PAGE followed by silver staining. ((b), (c), and (d)) Human Hb was incubated with oxLDL (50–400 μg/mL) for 90 minutes. ((b) and (c)) Spectral scan of Hb samples were taken and concentrations of Hb and metHb were calculated based on the visible spectra. (d) Hb samples (4 μg/lane) were subjected to SDS-PAGE followed by silver staining. Silver staining images and spectral scan are representatives of three independent experiments. Results are shown as mean ± S.D. () from one representative experiment of three.
