Research Article

Nitroglycerine-Induced Nitrate Tolerance Compromises Propofol Protection of the Endothelial Cells against TNF-α: The Role of PKC-β2 and NADPH Oxidase

Figure 4

Effects of TNF-α, nitroglycerine and CGP53353 on NOx (a) and nitrotyrosine production (b). Primary cultured human umbilical vein endothelial cells (HUVECs) were either not treated (control, Con.) or treated with TNF-α (40 ng/mL) alone (T) or with TNF-α in the presence of nitroglycerine (10 μM) (T + N), propofol (100 μM) (T + P), CGP53353 (1 μM) (T + C), propofol plus nitroglycerin (T + N + P), or CGP53353 plus nitroglycerine (T + N + C), respectively, for 24 h. All results are expressed as mean ± S.E.M., , (a) * compared with Con., T + N, T + P, T + N + P and T + C, ** compared with T + N, T + P and T + N + P; (b) * compared with Con., T + N, T + P, T + C and T + N + C, ** compared with Con., T + P, T + C and T + N + C.
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(a)
678484.fig.004b
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