Accelerated Aging during Chronic Oxidative Stress: A Role for PARP-1

Figure 3

(a) PAR polymer formation in human fibroblasts treated with 300 μM H₂O₂ for 10 minutes in the presence or absence of 1 μM fisetin or 100 nM minocycline which were added 30 minutes before the H₂O₂ treatment. **𝑃<0.05 compared to cells treated with 300 μM H₂O₂. (b) Representative photographs of PAR polymer staining. Arrows indicate examples of PAR polymer positive cells. Magnification: 300x.

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