Research Article

Inhibition of Nuclear Nox4 Activity by Plumbagin: Effect on Proliferative Capacity in Human Amniotic Stem Cells

Figure 1

Immunofluorescence analysis of Nox4 expression and nuclear ROS in AFSC. (a) Representative images showing superimposing between DAPI (blue) and Nox4 (green) signals obtained with antibody from Novus (Nov), Abcam (Abc), and Santa Cruz (SC). Inside all the three images, a square showing magnification of a cell with only the Nox4 signal in green is present. The highest magnification of one nucleus is shown for Santa Cruz labelling. (b) Representative images showing staining with nuclear ROS probe (Nuclear peroxy Emerald 1). Scale bar: 10 μm.
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