Inhibition of Nuclear Nox4 Activity by Plumbagin: Effect on Proliferative Capacity in Human Amniotic Stem Cells
Nox4 localisation during culture passages. (a) Cytofluorimetric analysis with propidium iodide (PI) of AFSC in culture from passage 1 to 12. Representative FACS analysis of cells at passages 6 and 12. The graph shows the percentage of cells on S phase. (b) Representative images of Western blot analysis of cytosol and nuclei of AFSC from passages 7 to 12 and revealed with Nox4 from Novus, Cyclin E, and actin. Presented data are representative of three independent experiments. (c) Representative images showing superimposing between DAPI (blue) and Nox4 SC (green) signals of AFSC cultures at passages 8, 10, and 12. Arrows indicate Nox4 staining in the nuclei. Scale bar: 10 μm.
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