Inhibition of Nuclear Nox4 Activity by Plumbagin: Effect on Proliferative Capacity in Human Amniotic Stem Cells

Figure 7

Effect of plumbagin on Nox4 nuclear interaction. (a)  Representative images of Western blot analysis of total lysates of AFSC treated with 2 μM plumbagin or 1 μM DPI for 24 hours. Membranes were revealed with anti-Nox4, anti-PERK1/2, and antiactin. (b) Subcellular fractions obtained with the plumbagin treatment were prepared for immunoprecipitation analysis. Nuclear lysate (NL) were immunoprecipitated with Nox4 antibody from Novus then revealed with anti-Matrin, anti-Nox4, and anti-PERK1/2. (c) Nuclear lysate (NL) were immunoprecipitated with anti-14-3-3 then revealed with anti-Nox4 and anti-14-3-3. All presented data are representative of three independent experiments. (d) Representative images showing superimposing between Matrin 3 (green) and Nox4 (red) signals of AFSC in the presence or absence of 2 μM plumbagin for 72 hours. Scale bar: 10 μm.