Research Article

Protective and Antioxidant Effects of a Chalconoid from Pulicaria incisa on Brain Astrocytes

Figure 4

Pulichalconoid B attenuates H2O2-induced ROS production in astrocytes. Astrocytes were preloaded with DCF-DA for 30 min and washed. Preloaded astrocytes were then preincubated with pulichalconoid B or quercetin for 2 h. H2O2 was added to the culture and the fluorescence intensity representing ROS levels were measured. (a) The fluorescence levels (Fluorescence units, FU) of cells that had been treated with 175 μM H2O2. ROS levels were measured at the indicated time points. (b) The ROS levels of cells that had been preincubated with various concentrations of pulichalconoid B or quercetin before H2O2 addition were measured after 1 h. The results represent the means ± SEM of two experiments ( ). Statistical analyses were performed with one-way ANOVA followed by the Tukey-Kramer multiple comparison tests ** , *** .
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