Protective and Antioxidant Effects of a Chalconoid from Pulicaria incisa on Brain Astrocytes
Figure 6
Pulichalconoid B inhibits peroxyl radical-induced oxidation of DCFH to DCF in primary astrocytes. Astrocytes were incubated with pulichalconoid B. Then, the astrocytes were preloaded with DCF-DA for 30 min and washed. ABAP (0.6 mM) was added to the culture, and the fluorescence intensity (representing ROS levels) was measured. (a) The fluorescence levels of cells that had been pre-incubated with 37.5 μM pulichalconoid B were measured at the indicated time points. The results are the means ± SEM of three experiments (). (b) The fluorescence levels of cells that have been pre-incubated with various concentrations of pulichalconoid B were measured after 20 h. The results are the mean ± SEM of three experiments (). “W/o” means without. Statistical analyses were performed with one-way ANOVA followed by the Tukey-Kramer multiple comparison tests. ***.
