TY - JOUR A2 - Shi, Honglian AU - da Silva, Jucélia Barbosa AU - Temponi, Vanessa dos Santos AU - Gasparetto, Carolina Miranda AU - Fabri, Rodrigo Luiz AU - Aragão, Danielle Maria de Oliveira AU - Pinto, Nícolas de Castro Campos AU - Ribeiro, Antônia AU - Scio, Elita AU - Del-Vechio-Vieira, Glauciemar AU - de Sousa, Orlando Vieira AU - Alves, Maria Silvana PY - 2013 DA - 2013/12/31 TI - Vernonia condensata Baker (Asteraceae): A Promising Source of Antioxidants SP - 698018 VL - 2013 AB - The present study evaluated the antioxidant potential of Vernonia condensata Baker (Asteraceae). Dried and powdered leaves were exhaustively extracted with ethanol by static maceration followed by partition to obtain the hexane, dichloromethane, ethyl acetate, and butanol fractions. Total phenols and flavonoids contents were determined through spectrophotometry and flavonoids were identified by HPLC-DAD system. The antioxidant activity was assessed by DPPH radical scavenging activity, TLC-bioautography, reducing power of Fe+3, phosphomolybdenum, and TBA assays. The total phenolic content and total flavonoids ranged from 0.19 to 23.11 g/100 g and from 0.13 to 4.10 g/100 g, respectively. The flavonoids apigenin and luteolin were identified in the ethyl acetate fraction. The IC50 of DPPH assay varied from 4.28 to 75.10 µg/mL and TLC-bioautography detected the antioxidant compounds. The reducing power of Fe+3 was 19.98 to 336.48 μg/mL, while the reaction with phosphomolybdenum ranged from 13.54% to 32.63% and 56.02% to 135.00% considering ascorbic acid and rutin as reference, respectively. At 30 mg/mL, the ethanolic extract and fractions revealed significant effect against lipid peroxidation. All these data sustain that V. condensata is an important and promising source of bioactive substances with antioxidant activity. SN - 1942-0900 UR - https://doi.org/10.1155/2013/698018 DO - 10.1155/2013/698018 JF - Oxidative Medicine and Cellular Longevity PB - Hindawi Publishing Corporation KW - ER -