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Oxidative Medicine and Cellular Longevity
Volume 2013, Article ID 698018, 9 pages
http://dx.doi.org/10.1155/2013/698018
Research Article

Vernonia condensata Baker (Asteraceae): A Promising Source of Antioxidants

1Graduate Program in Pharmaceutical Sciences, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil
2Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil
3Department of Biochemistry, Institute of Biological Sciences, Federal University of Juiz de Fora, Campus Universitário, 36036-330 Juiz de Fora, MG, Brazil

Received 19 August 2013; Accepted 27 November 2013

Academic Editor: Honglian Shi

Copyright © 2013 Jucélia Barbosa da Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study evaluated the antioxidant potential of Vernonia condensata Baker (Asteraceae). Dried and powdered leaves were exhaustively extracted with ethanol by static maceration followed by partition to obtain the hexane, dichloromethane, ethyl acetate, and butanol fractions. Total phenols and flavonoids contents were determined through spectrophotometry and flavonoids were identified by HPLC-DAD system. The antioxidant activity was assessed by DPPH radical scavenging activity, TLC-bioautography, reducing power of Fe+3, phosphomolybdenum, and TBA assays. The total phenolic content and total flavonoids ranged from 0.19 to 23.11 g/100 g and from 0.13 to 4.10 g/100 g, respectively. The flavonoids apigenin and luteolin were identified in the ethyl acetate fraction. The IC50 of DPPH assay varied from 4.28 to 75.10 µg/mL and TLC-bioautography detected the antioxidant compounds. The reducing power of Fe+3 was 19.98 to 336.48 μg/mL, while the reaction with phosphomolybdenum ranged from 13.54% to 32.63% and 56.02% to 135.00% considering ascorbic acid and rutin as reference, respectively. At 30 mg/mL, the ethanolic extract and fractions revealed significant effect against lipid peroxidation. All these data sustain that V. condensata is an important and promising source of bioactive substances with antioxidant activity.