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Oxidative Medicine and Cellular Longevity
Volume 2013, Article ID 704654, 7 pages
Research Article

Potentiation of Antibiofilm Activity of Amphotericin B by Superoxide Dismutase Inhibition

1Centre of Microbial and Plant Genetics, KU, Leuven, 3001 Heverlee, Belgium
2CMPG, Kasteelpark Arenberg 20, 3001 Heverlee, Belgium

Received 8 May 2013; Accepted 23 July 2013

Academic Editor: Cristina Mazzoni

Copyright © 2013 Katrijn De Brucker et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study demonstrates a role for superoxide dismutases (Sods) in governing tolerance of Candida albicans biofilms to amphotericin B (AmB). Coincubation of C. albicans biofilms with AmB and the Sod inhibitors N,N′-diethyldithiocarbamate (DDC) or ammonium tetrathiomolybdate (ATM) resulted in reduced viable biofilm cells and increased intracellular reactive oxygen species levels as compared to incubation of biofilm cells with AmB, DDC, or ATM alone. Hence, Sod inhibitors can be used to potentiate the activity of AmB against C. albicans biofilms.