Table 1: FoxO regulation by Sirt1.

Cell cultureTreatmentOutcomeReference

HEK 293TStress conditions (H2O2)↑ Interaction between Sirt1 and FoxO3
MEF Sirt1−/−Treatment with LY 294002 (PI3K inhibitor): wild type Sirt1−/−↑ Acetylation of FoxO3
↑ GADD45 (stress resistance)
HepG2Serum starvation => FoxO translocation into the nucleus↑ Deacetylation of FoxO1 by Sirt1[55]
HeLaSirt1 overexpression FoxO3 activity
( FoxO3 target genes Bim/p27)
HEK 293TInhibition of Sirt1 FoxO4 activity

Bim: Bcl2 interacting mediator of cell death, proapoptotic protein; H2O2: hydrogen peroxide; HEK-293T: human embryonic kidney 293 cells containing the T antigen from simian virus; HepG2: human liver carcinoma cell line; MEF: mouse embryonic fibroblasts; MnSOD: manganese superoxide dismutase, part of antioxidative defence; p27: cyclin-dependent kinase inhibitor, controls cell cycle progression; PI3K: phosphatidylinositol 3-kinase.