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Oxidative Medicine and Cellular Longevity
Volume 2013, Article ID 741817, 9 pages
http://dx.doi.org/10.1155/2013/741817
Research Article

The Potential Role of Azadirachta indica Treatment on Cisplatin-Induced Hepatotoxicity and Oxidative Stress in Female Rats

1Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
2Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo 11795, Egypt
3Biological Science Department, Faculty of Dentistry, Modern Science and Arts University (MSA), Giza 12111, Egypt
4Biochemistry and Molecular Biology Department, Faculty of Pharmacy, Modern Science and Arts (MSA), Giza 12111, Egypt
5Molecular Drug Evaluation Department, National Organization for Drug Control & Research (NODCAR), Giza 12553, Egypt
6Department of Biochemistry and Molecular Biology, Asturias Institute of Biotechnology, University of Oviedo, Oviedo 33006, Spain

Received 6 October 2013; Revised 6 November 2013; Accepted 17 November 2013

Academic Editor: Vladimir Jakovljevic

Copyright © 2013 Mohamed A. Dkhil et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Azadirachta indica A. Juss. (neem, family: Meliaceae) is perhaps the most commonly used traditional medicinal plant of India. In this study we investigated the protective effect of methanolic neem leaves extract (MNLE; 500 mg/Kg bwt) on rats treated with cisplatin (CDDP)-induced hepatotoxicity. Adult rats were randomly divided into four groups. CDDP was given to rats by intraperitoneal injection, while MNLE was given by oral gavage for 5 days after the CDDP injection. The injury and oxidative stress caused by CDDP on the liver and the effect of MNLE were evaluated by measuring (a) histological changes, (b) tissue biochemical oxidant and antioxidant parameters, and (c) investigating apoptosis markers immunohistochemically and by real time PCR. After treatment with MNLE, the histological damage and apoptosis induction caused by cisplatin were improved. Malondialdehyde and nitric oxide were significantly decreased; the antioxidant system, namely, glutathione content, glutathione-S-transferase, glutathione peroxidase, catalase, and superoxide dismutase activities were significantly elevated. In conclusion, MNLE may have a potential role when combined with cisplatin in chemotherapy to alleviate cisplatin-induced damage and oxidative stress in liver.