Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 6

Research Article

Major Components of Energy Drinks (Caffeine, Taurine, and Guarana) Exert Cytotoxic Effects on Human Neuronal SH-SY5Y Cells by Decreasing Reactive Oxygen Species Production

Figure 6

Qualitative analysis of drug-induced changes on cellular viability. Confocal immunofluorescence microscopy (CM) of propidium iodide (PI) (red fluorescence), β-III-tubulin expression (green fluorescence), and DAPI (nuclei) on human neuronal SH-SY5Y cells (a), after treatment with 12.5 mg/mL of guarana (b), 0.5 mg/mL of caffeine (c), and 4 mg/mL of taurine (d) for 4 hours in FBS-free culture medium. Scale bars represent 10 μm.