Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 6

Research Article

The NRF2 Activation and Antioxidative Response Are Not Impaired Overall during Hyperoxia-Induced Lung Epithelial Cell Death

Figure 6

NQO1 promoter activity in AECs exposed to hyperoxia acutely and chronically. To determine the effects of hyperoxia on NQO1 promoter activity, luciferase reporter construct bearing NQO1 promoter (100 ng) and pRL-TK coding for the Renilla luciferase were transiently transfected into AECs, exposed to hyperoxia or room air, and luciferase activity was analyzed using dual luciferase assay kit. The promoter activity of AECs exposed to room air was set as on unit. Values represent the average of 3 independent experiments done in triplicate ( ).

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