Curcumin Pretreatment Induces Nrf2 and an Antioxidant Response and Prevents Hemin-Induced Toxicity in Primary Cultures of Cerebellar Granule Neurons of Rats
Figure 1
Hemin induced neuronal death and reactive oxygen species (ROS) production in cerebellar granule neurons (CGNs). Cultures were exposed to hemin for 1 h followed by recovery in growth medium for 24 h. The data were obtained after this time. Viability was assessed by (a) fluorescein diacetate (FDA) fluorescence and (b) 3-[4,5-dimethylthiazol-|2-yl)]-2,5-diphenyl-tetrazolium bromide (MTT) reduction. (c) Pearson correlation index between FDA and MTT assays. (d) ROS production was evaluated after 1 h of incubation with 30 μM hemin. Bright-field (left panel, H: hemin), 5-(and 6-)carboxy-2′,7′-dichlorofluorescein (carboxy-DCF, middle panel), and ethidium (right panel). The same field is shown in each condition. (e) Intensity of carboxy-DCF or ethidium fluorescence was measured in five different fields per well per condition and was quantified using CGNs with the respective probe as a control. Data are expressed as mean ± SEM, = 3–5. versus 0 μM hemin.
