Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 3

Research Article

Ethanol and Acetate Acting as Carbon/Energy Sources Negatively Affect Yeast Chronological Aging

Figure 3

The glyoxylate-requiring gluconeogenesis plays a positive role in extreme life-span extension. At Day 1 after the diauxic shift, wt cells were switched to water adjusted to pH 3.2 (a) and to pH 5.6 (b) and challenged with ethanol (6 g/L), acetic acid (5 mM), or both. Every 48 h, cultures were resuspended in fresh water, and each time ethanol and acetic acid were added when indicated. At every time-point, viability was measured. Survival of cells in their expired medium was also monitored as control. Insets: CLS plotted on a log scale. One representative experiment is shown. (c) CLS of wt, icl1Δ, pck1Δ, and sir2Δ cells switched to pH 3.2 water at Day 1 after the diauxic shift. (d) In parallel, the indicated cultures were challenged with 5 mM acetic acid as in (a). Survival of icl1Δ cells in their exhausted medium was also monitored. One representative experiment is shown.
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(a)
802870.fig.003b
(b)
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(c)
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(d)

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