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Oxidative Medicine and Cellular Longevity
Volume 2013, Article ID 859056, 9 pages
Research Article

Transient Limb Ischemia Alters Serum Protein Expression in Healthy Volunteers: Complement C3 and Vitronectin May Be Involved in Organ Protection Induced by Remote Ischemic Preconditioning

1Department of Anesthesiology, The Sixth Affiliated Hospital, Sun Yat-sen University, No. 26 Yuancunerheng Road, Guangzhou 510655, China
2Department of Anatomy, Medical College of Jinan University, No. 601 West Huangpu Avenue, Guangzhou 510632, China

Received 5 September 2013; Accepted 30 September 2013

Academic Editor: Zhengyuan Xia

Copyright © 2013 Ting Pang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The protective mechanism underlying remote ischemic preconditioning (RIPC) is unclear. This study aims to verify whether the protein expression profile in the serum could be altered by RIPC and to detect potential protein mediators. Transient limb ischemia consisting of three cycles of 5-min ischemia followed by 5-min reperfusion was performed on sixty healthy volunteers. Serum samples were collected at 30 min before transient limb ischemia and at 1 hour (h), 3 h, 8 h, 24 h, and 48 h after completion of three cycles. Changes in the serum protein profile were analyzed by two-dimensional gel electrophoresis and proteins were identified by MALDI-TOF/TOF mass spectrometry. Fourteen differentially expressed proteins were identified and, respectively, involved in immune system, lipid binding and metabolism, apoptosis, and blood coagulation. Complement C3, vitronectin, and apolipoprotein A-I were further confirmed by western blotting, and the results showed that their contents decreased significantly after transient limb ischemia. It is concluded that transient limb ischemia alters the serum protein expression profile in human being, and that reduction of serum contents of complement C3 and vitronectin may represent an important part of the mechanism whereby RIPC confers its protection.