Transient Limb Ischemia Alters Serum Protein Expression in Healthy Volunteers: Complement C3 and Vitronectin May Be Involved in Organ Protection Induced by Remote Ischemic Preconditioning
Table 2
Differentially expressed proteins in sera after RIPC compared with that in sera before RIPC.
Spota
Protein name
Accession numberb
Protein MW (Da)
Protein PIc
Sequence coveraged (%)
Fold changee
Protein scoref
(i)
Immune system process
1
Complement component 4B
IPI00887154
192627.5
6.89
2
↓1.951 h
158
2
Complement C1q subcomponent subunit B
IPI00477992
26704.5
8.83
22
↓2.733 h
84
3
Complement C3
IPI00783987
187029.9
6.02
15
↓2.231 h
121
4
C4b-binding protein alpha chain
IPI00021727
66989.4
7.15
9
↓1.511 h
71
5
Ficolin-3
IPI00419744
31657.4
6.36
18
↑1.521 h
107
6
Interalpha-trypsin inhibitor heavy chain H4
IPI00896419
103293
6.51
18
↑2.511 h
97
7
Vitronectin
IPI00298971
54271.2
5.55
4
↓1.521 h
70
(ii)
Lipid metabolic process
8
Apolipoprotein A-I
IPI00021841
30758.9
5.56
25
↓3.851 h
79
9
Apolipoprotein L1
IPI00514475
43946.9
5.6
28
↑1.681 h
88
10
Apolipoprotein J
IPI00291262
52461
5.89
20
↓1.7424 h
219
(iii)
Apoptosis
11
Desmoplakin
IPI00013933
331568.7
6.44
8
↑1.6248 h
67
12
Gelsolin
IPI00026314
85644.2
5.9
16
↑2.211 h
131
(iv)
Blood coagulation
13
Antithrombin-III
IPI00032179
52657.8
6.11
23
↓2.011 h
180
14
Heparin cofactor 2
IPI00879573
57034.2
6.41
17
↑2.321 h
64
Spot number refers to Figure 1.
bAccession number from IPI (International Protein Index) database of matched proteins.
cPI refers to isoelectric point.
dPercent of number of observed amino acids in sequence length (%).
e“↓” means downregulation, and “↑” means upregulation. Superscripts such as “1h” represented the time point when the fold change reached maximum. The fold change in the table was the maximum change among the different time points.
fCombined scores of all observed mass spectra matched to amino acid sequences used for protein identification.