Brazilian Green Propolis Suppresses the Hypoxia-Induced Neuroinflammatory Responses by Inhibiting NF-κB Activation in Microglia
Figure 1
Effect of propolis on hypoxia-induced mitochondria ROS associated cytotoxicity in cultured microglia. (a) Cell viability in MG6 microglia in the presence of propolis with different three concentrations. (b) Cell viability of MG6 microglia exposed to normoxia (20% O2) or hypoxia (1% O2) in the presence or absence of propolis (50 μg/mL) for 24 h. Each column and bar represent mean ± SEM ( each). An asterisk indicates a statistically significant difference from the value in Normoxia (*). A sword indicates a statistically significant difference from the value in hypoxia (†). (c) Fluorescent mages of MitoSOX Red fluorescence signals in MG6 microglia exposed to normoxia (20% O2) or hypoxia (1% O2) in the presence or absence of propolis (50 μg/mL) for 24 h. Scale bar = 10 μm. (d) The quantitative analyses of MitoSOX Red fluorescence signal intensity in (c). Each column and bar represent the mean ± SEM ( each). An asterisk indicates a statistically significant difference from the value in normoxia (*). A sword indicates a statistically significant difference from the value in hypoxia (†).
