Research Article

Thiol Redox Sensitivity of Two Key Enzymes of Heme Biosynthesis and Pentose Phosphate Pathways: Uroporphyrinogen Decarboxylase and Transketolase

Table 2

SRM quantification of Hem12p and Tkl1p in WT and ΔGrx2 yeast strains. Proteotypic peptides and transitions used for SRM analysis and relative quantification are shown. Peak intensity is given in arbitrary units ×104. SRM was performed with two independent biological replicates.

Proteotypic peptideQ1 ( )Q3 ( )FragmentationPeak intensity
WTΔGrx2

Uroporphyrinogen decarboxylase
 VTLQGNLDPGVMYGSK839.93838.412+/y82.351.66
 DAEIASEITIQPVR771.411113.632+/y101.87ND
 YIVNFGHGTHPFMDPDVIK1094.041061.532+/y93.32ND
 NPEDLQTVLDYK717.86979.552+/y85.166.33
 QMIEAFGGGK519.26665.332+/y72.42ND
Transketolase
 FFGFTPEGVAER678.83757.392+/y79.704.10
 QNLPQLEGSSIESASK844.431332.662+/y130.630.18
 SFVVPQEVYDHYQK580.29653.812+/y109.416.63
 ANSGHPGAPLGMAPAAHVLWSQMR819.41919.492+/y70.220.23
 SLPNIQVWRPADGNEVSAAYK772.40938.462+/y90.280.21

ND not detected.