Activation of the Nrf2 Pathway by Inorganic Arsenic in Human Hepatocytes and the Role of Transcriptional Repressor Bach1
Figure 1
NaAsO2 increases total cellular Nrf2 protein levels in Chang human hepatocytes. Whole-cell protein extracts were separated by SDS-PAGE, and western blot analysis was conducted using antibodies of Nrf2 and β-actin, respectively. (a) Cells were treated with 10 μmol/L of NaAsO2 for 2, 4, 6, 12, 24, and 36 h. (b) Cells were treated with 25 μmol/L of NaAsO2 for 2, 4, 6, 12, and 24 h. (c) Cells were treated with 50 μmol/L of NaAsO2 for 2, 4, 6, 12 and 24 h. (d) Cells were treated with NaAsO2 (10, 25, and 50 μmol/L) for 6 h. (a), (b), (c), and (d) showed representative immunostained bands from three independent cultures. β-actin was used as an internal control to ensure equal loading in all lanes of the gel. The right column diagrams were quantitative analysis of (a), (b), (c), and (d) and finally expressed as ratio to control cells. Data were mean ± standard deviations (SD) from three different samples. versus control cells.
