Decitabine and SAHA-Induced Apoptosis Is Accompanied by Survivin Downregulation and Potentiated by ATRA in p53-Deficient Cells
(a) Effect of 1 μM DAC and/or 1 μM SAHA on survivin expression after 48 hours treatment. Error bars represent ±SEM and immunoblots from cell lysates of leukemic cell lines CML-T1 and HL-60 are representatives of at least five experiments with similar results. Statistical significance degree of difference between treated samples and the corresponding control: (#), (**), (***). (b) Untreated or DAC + SAHA-treated cells of HL-60 cell line were permeabilized and stained for survivin (Alexa Fluor 488, green) and DNA content (Hoechst 33342, blue). Fluorescence intensity distribution and intracellular localization were assessed by flow-cytometry and confocal microscopy. Scale bar represents 5 μm.