Oxidative Medicine and Cellular Longevity / 2014 / Article / Fig 4

Research Article

Decitabine and SAHA-Induced Apoptosis Is Accompanied by Survivin Downregulation and Potentiated by ATRA in p53-Deficient Cells

Figure 4

(a) Production of reactive oxygen species by control cells (black, full line) and HL-60 cells treated for 48 h by 1 μM ATRA (grey, full line), DAC + SAHA (black, dashed line), or DAC + SAHA + ATRA combination (grey, dashed line) monitored by flow-cytometric analysis of H2DCFDA staining. Embedded chart: mean value of flow-cytometer-detected fluorescence intensity was analyzed. (b) NAC for reduction of ROS generation or caspases inhibitors z-VAD-fmk or Q-VD-OPh were used in concentrations as indicated and ROS generation as well as fraction of cells in subG1 phase were measured by flow cytometry. Error bars represent ±SEM of three measurements. Statistical significance degree between corresponding samples was analyzed and value evaluated as (*), (**), (***).