PELP1 Suppression Inhibits Colorectal Cancer through c-Src Downregulation

Figure 2

PELP1 downregulation inhibited the CRC cell line HT-29 in vitro. (a) After transfection with shRNA, PELP1 protein expression was decreased by 90%. shRNA #3 was selected for further investigations. (b) After PELP1 silencing, the cell viability of HT-29 was inhibited. (c) PELP1 silencing inhibited the colony formation ability of HT-29 by 57.5% (lower panel). A representative colony formation assay is shown (upper panel). (d) PELP1 silencing inhibited the migration ability of HT-29 by 69.3% (lower panel). A representative migration assay is shown (upper panel). (e) PELP1 silencing inhibited the invasion ability of HT-29 by 58% (lower panel). A representative invasive assay is shown (upper panel). (f) Senescence was induced by PELP1 silencing in HT-29. A representative β-Gal assay is shown.