Oxidative Medicine and Cellular Longevity

Oxidative Medicine and Cellular Longevity / 2014 / Article
Special Issue

Nitric Oxide-related Oxidative Stress and Redox Status in Health and Disease

View this Special Issue

Clinical Study | Open Access

Volume 2014 |Article ID 203512 | 9 pages | https://doi.org/10.1155/2014/203512

L-Arginine/NO Pathway Is Altered in Children with Haemolytic-Uraemic Syndrome (HUS)

Academic Editor: Daniela Giustarini
Received06 Jan 2014
Accepted11 Feb 2014
Published17 Mar 2014


The haemolytic uraemic syndrome (HUS) is the most frequent cause of acute renal failure in childhood. We investigated L-arginine/NO pathway in 12 children with typical HUS and 12 age-matched healthy control subjects. Nitrite and nitrate, the major NO metabolites in plasma and urine, asymmetric dimethylarginine (ADMA) in plasma and urine, and dimethylamine (DMA) in urine were determined by GC-MS and GC-MS/MS techniques. Urinary measurements were corrected for creatinine excretion. Plasma nitrate was significantly higher in HUS patients compared to healthy controls , whereas urine nitrate was borderline lower in HUS patients compared to healthy controls . ADMA plasma concentrations were insignificantly lower, but urine ADMA levels were significantly lower in the HUS patients . Urinary DMA was not significantly elevated. In HUS patients, nitrate but not nitrite, L-arginine, or ADMA concentrations in plasma correlated with free haemoglobin concentration. Our results suggest that both NO production and ADMA synthesis are decreased in children with typical HUS. We hypothesize that in the circulation of children with HUS a vicious circle between the L-arginine/NO pathway and free haemoglobin-mediated oxidative stress exists. Disruption of this vicious circle by drugs that release NO and/or sulphydryl groups-containing drugs may offer new therapeutic options in HUS.

1. Introduction

Haemolytic-uraemic syndrome (HUS) is the most frequent cause of acute renal failure in childhood [1]. HUS is defined by the triad of haemolytic anaemia, acute renal failure, and thrombocytopenia. HUS in childhood is predominantly induced by an infection with verocytotoxin- (Shiga-like toxin-) producing bacteria, typically Escherichia coli. HUS primarily occurs in children one to 10 years of age with an average annual incidence of one to three cases per 100,000 children and a survival rate of nearly 95% [2]. Beside the outbreak of Shiga-toxin producing E. coli O104:H4 in 2011 [3], in Austria and Germany, the incidence is 0.4 : 100,000 and 0.7 : 100,000, respectively [4]. The disease begins after an incubation period of 4 to 7 days with abrupt onset of bloody diarrhoea and abdominal pain. Two to ten days later, microangiopathy, haemolytic anaemia, thrombocytopenia, and acute renal failure develop. Specific pathological findings in HUS patients with acute renal failure are glomerular microthrombi [5, 6]. HUS microangiopathy can involve almost any organ, but damage to kidneys and central nervous system cause the most severe clinical problems [7]. Despite the often dramatic clinical presentation, the overall outcome of childhood HUS is relatively good. Mortality is less than 5%, and 75% of patients show complete remissions [8]. Relapses are rare.

Shiga-like toxin binds to the glycosphingolipid globotriaosylceramide [9], thus interfering with protein synthesis in endothelial cells. Disordered von Willebrand factor, platelet activation via platelet-activating factor, interleukins, and nitric oxide (NO) may also contribute to the disorder [7]. NO has multiple functions including regulation of vascular tone, neurotransmission, and inhibition of platelet aggregation and leukocyte adhesion [10]. Antiproliferative and antiatherosclerotic effects have also been ascribed to NO [11].

NO is a short-lived free radical which is produced in all types of cells including endothelial cells. In vivo, NO is rapidly oxidized to nitrate and nitrite, which circulate in blood and are excreted in the urine [12]. Under certain conditions circulating nitrite and nitrate and excretory nitrate are suitable indicators and measures of NO synthesis [13]. Thus, nitrate in urine is considered a useful measure of whole body NO synthesis, whereas circulating nitrite rather reflects endothelium-dependent NO synthesis. NO is generated from the amino acid L-arginine by NO synthases (NOS) isoforms, including endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNOS). These enzymes oxidize the imino group of the terminal guanidine group of L-arginine to NO, with L-citrulline being the second reaction product [14].

NOS activity is regulated by endogenous inhibitors, with asymmetric dimethylarginine (ADMA) being the most important [15]. ADMA is generated by methylation of protein-associated L-arginine catalyzed by N-methyl protein transferases followed by regular proteolysis [16]. Unchanged ADMA is excreted by the kidneys, but the greatest part of endogenously produced ADMA (about 90%) is excreted in the urine as dimethylamine (DMA) after hydrolysis by the enzyme dimethylarginine dimethylaminohydrolase (DDAH) [15], predominantly in kidney and liver. High circulating ADMA levels were found in many diseases including chronic renal failure [17].

In adult patients suffering from different microangiopathic diseases NO synthesis seems to be increased [18]. Yet, in childhood data on the L-arginine/NO pathway is rare. We recently reported reference data for the different members of the NO family in childhood [19]. We also studied these parameters in children suffering from renal diseases [20]. As microangiopathy also occurs in HUS patients, we were interested to see if the L-arginine/NO pathway is altered in children with HUS. It is worth mentioning that in the acute phase of HUS, erythrocytes are exposed to elevated oxidative stress that could contribute to haemolysis directly through oxidative damage and/or decreasing membrane fluidity [21]. More recently, it was demonstrated that during the acute phase of HUS in humans oxidative stress is elevated when measured as lipid peroxidation products in plasma [22]. In mice, Shiga toxin 2 was shown to increase oxidative stress and antioxidants such as N-acetylcysteine were found to ameliorate Shiga toxin-induced oxidative stress measured as malondialdehyde and to decrease renal damage [23]. In a patient, a 9-year-old girl, suffering from HUS after transplantation transdermal application of isosorbide dinitrate (ISDN), a NO-releasing drug was found to be very effective in ameliorating haemolysis and in increasing the number of platelets [24]. This interesting finding indicates that NO may play a protective role in HUS. In consideration of the potential involvement of the L-arginine/NO pathway in children suffering from typical HUS, we wanted to characterize quantitatively and comprehensibly the status of HUS by measuring several members of the L-arginine/NO pathway in plasma and urine. Healthy children served as the control group in the present study. As nitrite in human urine may potentially indicate nitrative stress [25], we quantitated urinary nitrite in addition to urinary nitrate which is a measure of whole-body NO synthesis [13].

2. Materials and Methods

Twelve children with typical HUS (5 girls, 7 boys; mean age years) and twelve age-matched healthy controls were included. The clinical characteristics of the patients are summarized in Table 1. Patients were treated symptomatically with diuretics and dialysis. If arterial hypertension was present they received antihypertensive drugs (Table 1). Patients were discharged about two weeks after the end of dialysis which was performed for 5 to 30 days (Table 1). Blood and, where possible, urine were taken at the first or second day of inpatient stay. The study was approved by the Ethics Committee of the Hannover Medical School and written consent was obtained from the parents.

Patient numberDuration of peritoneal dialysis (days)Neurological eventsHypertensionSerum creatinine (µM)Serum urea (mM)Urine albumin (g/L)Total hemoglobin (g/dL)Free hemoglobin (mg/L)Lactate dehydrogenase (U/L)

10 NoNo10815.43.76.64292448
27 NoNo39428.40.377.6196968
45 NoNo45841.90.415.91652606
59 EEG alterationNo13220.4Not measured6.81551462
624 NoYes20921.00.895.7Not measured2611
730 Status epilepticusNo30426.30.244.8Not measured2593
810 NoNo507.84.456.9843090
98 EEG alterationNo52839.4Not measured13.0Not measured2003
106 NoNo60068.0Not measured8.2Not measuredNot measured
1120 NoYes37319.83.46.0Not measured2662
1214 EEG alterationNo29628.44.976.22842379

ADMA in plasma and urine and L-arginine in plasma were determined by GC-MS/MS and GC-MS, respectively, as described elsewhere [26]. Nitrite and nitrate in plasma and urine were determined simultaneously by GC-MS as described previously [27]. Urinary creatinine was determined by GC-MS [28]. DMA in urine was determined by GC-MS as described recently [29]. Urinary excretion of the analytes was corrected for creatinine excretion and data are presented as μmol of the analyte per mmol of creatinine.

As a measure of endothelial damage, circulating endothelial cells were detected in blood at the time of admission as described by us elsewhere [30].

Data from patients and healthy controls were compared using the Mann-Whitney test (SPSS, version 16). Data are presented as mean ± SD. Values of were considered significant.

3. Results

Upon admission, the number of circulating endothelial cells in the HUS childrens’ blood was 44 per mL (median, range 0 to 800 per mL), indicating endothelial damage [30]. At time of discharge, the number of circulating endothelial cells decreased to 24 per mL (median, range 0 to 180 per mL).

In children with HUS, plasma nitrate ( versus μM, ) and plasma nitrite ( versus μM, ) were significantly increased, as compared to the healthy controls (Figure 1). Excretion rates of nitrate were insignificantly lower in the HUS patients as compared to the healthy controls ( versus μmol/mmol, ) (Figure 2(a)). Urinary nitrite did not differ between HUS and healthy controls ( versus μmol/mmol, ) (Figure 2(b)).

Plasma ADMA concentrations were insignificantly lower in the HUS children as compared to the healthy children ( versus  nM, ) (Figure 3(a)). However, renal excretion rate of ADMA was significantly lower in patients with HUS compared to healthy controls (, , versus , ) μmol/mmol, ) (Figure 3(b)). Excretion rate of DMA in urine was insignificantly higher in patients with HUS, that is, () in comparison to () μmol/mmol in healthy children () (Figure 4). Plasma L-arginine plasma levels were not statistically different between the two groups (Figure 5; ).

In the HUS patients, there was a close positive correlation (, ) between plasma nitrate concentration () and plasma free haemoglobin concentration () with the regression equation ; by contrast, plasma nitrite, L-arginine, and ADMA concentration did not correlate with plasma free haemoglobin concentration (Figure 6).

4. Discussion

The L-arginine/NO pathway plays an important role in renal failure, infection, and microangiopathy [18, 31, 32]. In adult patients with microangiopathy endogenous NO production seems to be elevated [18]. In the present study we investigated the status of the L-arginine/NO pathway in children with typical haemolytic uraemic syndrome (HUS), which is associated with haemolytic anaemia, acute renal failure, and thrombocytopenia.

The number of free circulating endothelial cells in children with HUS at the time of admission was much higher than that commonly found in healthy individuals [30]. These expected findings suggest that endothelial damage occurred in the HUS patients investigated in the present study. At time of discharge, the number of circulating endothelial cells decreased to normal levels, indicating improvement of endothelial dysfunction.

To study the L-arginine/NO pathway we obtained blood and urine samples at the first or second day of inpatient stay. In the plasma samples of our patients with typical HUS we observed significantly increased concentrations of nitrate and nitrite compared to healthy children. In former studies of our groups, nitrate and nitrite concentrations were not elevated in children with syndromic [33] and nonsyndromic focal-segmental-glomerulosclerosis (FSGS) or in children with non-FSGS renal diseases [20]. Taken all together, impaired renal function in HUS patients is likely to be a reason for accumulating plasma nitrite and nitrate concentrations rather than an enhanced NO synthesis (Figure 7). In the children of the present study, we did not measure cGMP, the second messenger of NO, in plasma or urine samples. In forthcoming studies, measurement of cGMP in plasma of HUS children could provide additional, valuable information about NO biosynthesis/bioavailability in this syndrome, although circulating or urinary cGMP and NO synthesis or bioactivity are not dependable correlates.

Unfortunately, urine samples were not available from all HUS children and the power of the biochemical parameters measured in urine samples is considered rather low. While plasma ADMA concentrations were not increased in the HUS patients, urinary excretion rate of ADMA in HUS patients was almost threefold lower than in healthy children. Analogous to urinary nitrate, excretion of unchanged ADMA in the urine seems to be impaired most likely due to renal failure. These results and the observation that DMA excretion rates were similar in the HUS patients and the healthy controls suggest that ADMA synthesis rate is decreased in typical HUS, presumably due to impaired activity of N-methyl protein transferases and/or impaired activity of proteolytic enzymes in this syndrome (Figure 7). On the other hand, our results suggest that DDAH activity is not altered in HUS. Deficiency of L-arginine, the common precursor of NO and ADMA, is unlikely to explain the reduction in ADMA synthesis in the HUS patients, as plasma L-arginine concentrations were similar in patients and in control subjects.

Elevated NO production has been observed in a mouse model of Shiga toxin 2-induced HUS [34]. Shao et al. [35] suggested eNOS upregulation in a rat model for thrombotic microangiopathy and that elevated endothelial NO synthesis could be an important protective mechanism in thrombotic microangiopathy [35]. NO production by neutrophil leucocytes following stimulation with Shiga toxin has been found to be age-dependent with lower production in infants; the authors speculate that this may be related to the higher incidence in infants [36]. As nitrite and nitrate in plasma and/or in urine do not reflect the activity of a particular NOS isoform or organ, our results do not allow the drawing of any conclusions regarding eNOS contribution to NO in the HUS patients of healthy children. Therefore, in our HUS patients we cannot exclude elevation in expression and activity of iNOS which generates NO for purpose of antimicrobial defense, but we consider it rather very moderate.

The intact erythrocyte plays an important role both in NO inactivation through oxyhaemoglobin and in storage and transport of NO bioactivity within the circulation [3742]. Therefore, it is likely that haemoglobin species inside as well as outside of the erythrocyte, that is, free haemoglobin, are of particular importance for NO-related biological actions in HUS patients, in a way independent of the endothelial function. Free haemoglobin in plasma may act both as a trap for NO and as a producer of NO from inorganic nitrite, the autoxidation product of NO (Figure 7). Hypoxia is not atypical for HUS. Disturbed microcirculation in HUS caused by thrombotic microangiopathy may lead to hypoxia which facilitates nitrite reduction to NO by free haemoglobin. In this situation, additionally produced NO from nitrite may be meaningful and beneficial by acting as inhibitor of aggregation as well as a vasodilatator. In addition, haemoglobin may produce vasoactive substances such as prostaglandins and thromboxane, but it may also induce oxidative stress [43]. In the present study, we found a positive correlation between nitrate and free haemoglobin in plasma. This observation together with the almost uniform distribution of nitrite and nitrate in blood cells including erythrocytes [44, 45] may argue for oxidation of NO to nitrate by free haemoglobin outside of the erythrocyte rather than for nitrate release by damaged erythrocytes. It is worthy of mention that haemolysis in our HUS patients was not higher than about 0.2%. However, because free haemoglobin in the plasma is several times more reactive against NO than haemoglobin inside of the erythrocytes, it is likely that a large fraction of NO has been oxidized to nitrate by free haemoglobin in the plasma of our HUS patients. In HUS, like in sickle cell anemia [41], haemolysis seems to be associated with harmful rather than with beneficial effects. Cell-free haemoglobin released by haemolysis may oxidize NO to biologically inactive nitrate as mentioned above. In addition, free haemoglobin may also inactivate NO by enhancing oxidative stress, for instance, by producing reactive oxygen species (ROS) such as superoxide radical anions and hydrogen peroxide. Such ROS are highly reactive and may therefore oxidize NO to peroxynitrite. This is a strong oxidant on its own and will finally decompose to nitrite, nitrate and dioxygen. It is likely that these deleterious effects of haemoglobin are not exerted in a stoichiometric manner [43].

Based on our results suggesting a decreased synthesis rate of endogenous NO in HUS, one may speculate that basal NO synthesis is insufficient to ameliorate thrombotic microangiopathy in this syndrome and that exogenous NO in pharmacological doses may therefore be required. In a 9-year-old girl suffering from HUS following bone marrow transplantation at the age of 8 months, 20 mg/day transdermal ISDN for 9 weeks ameliorated haemolysis while increasing platelet counts. The girl did not experience side effects or the disease did not recur after cessation of ISDN treatment [24]. Interestingly, many of the clinical characteristics of this girl were comparable to those of our HUS patients. Unfortunately, no data had been reported about the L-arginine/NO pathway in this girl. It is worth mentioning that ISDN was found not to increase oxidative stress in healthy young subjects when applied at a therapeutically relevant dose of 30 mg thrice a day [46]. In contrast, at this dose ISDN appeared to decrease basal nitrative stress one day after administration when measured both as soluble 3-nitro-tyrosine and 3-nitro-tyrosine-albumin, though not statistically significant [46], which are potential biomarkers for NO-dependent oxidative stress in humans [47]. Due to the limited availability of urine samples and the artefactual contribution of haemolysis to biomarkers of oxidative stress such as malondialdehyde (MDA) and the F2-isoprostane 15(S)-8-iso-PGF2α [43], but not to ADMA and other members of the L-arginine/NO pathway [48], we abandoned the analysis of MDA and 15(S)-8-iso-PGF2α in the plasma and urine samples of our study.

In adults CAD patients we found that low urinary ADMA excretion rates are associated with impaired cardiac function and predict cardiovascular as well as all-cause mortality [49]. In the present study we measured in HUS children for the first time lower ADMA excretion rates than in healthy children. The potential clinical value of urinary ADMA for the diagnosis of CAD or cardiac dysfunction in adults and for the diagnosis of HUS in childhood warrants further studies.

In summary, the L-arginine/NO pathway is altered in childhood HUS. NO synthesis seems to be diminished despite a decreased synthesis of ADMA, an endogenous inhibitor of NO synthesis from L-arginine, compared to healthy age-matched children. Free haemoglobin is likely to play an important role in the metabolism and biological activity of NO, including endothelium-derived NO, and to induce oxidative stress, for instance, lipid peroxidation in blood, thus damaging the erythrocyte membrane and causing haemolysis. Further studies are required to delineate the relative contribution of damaged erythrocytes and damaged endothelium to HUS. Application of drugs with the potential to release NO, such as the organic nitrate ISDN, could be effective as a therapeutic means in the treatment of HUS. The underlying mechanism leading to impaired NO synthesis in HUS is unclear and demands further elucidation. The potential beneficial effect of organic nitrates on haemolysis and platelets and the underlying mechanism(s) warrant further investigations. It appears that oxidative stress is elevated in HUS while NO synthesis is decreased. Supplementation of children suffering from HUS with NO-releasing drugs or drugs that increase NO synthesis and/or bioavailability and that decrease oxidative stress, such as N-acetylcysteine (NAC) or its more lipophilic and cell membrane-permeable N-acetylcysteine ethyl ester (NACET) [50], may be useful measures among others in the treatment of HUS.

Conflict of Interests

The authors declare that they have no conflict of interests regarding the publication of this paper.

Authors’ Contributions

Thomas Lücke designed the study. Nele Kirsten Kanzelmeyer investigated blood and urine samples and wrote the paper. Hans Hartmann evaluated neurological outcome of patients and participated in writing the paper. Lars Pape and Anne-Jule Fuchs helped to evaluate patients. Kristine Chobanyan-Jürgens investigated DMA in urine. Dimitrios Tsikas established methods to measure members of the L-arginine/NO pathway. Bernhard Vaske did the statistics. Anibh Martin Das and Jens Jordan reviewed the paper. Marion Haubitz measured circulating endothelial cells.


The authors thank B. Beckmann and A. Mitschke for excellent laboratory assistance and F.-M. Gutzki for performing GC-MS and GC-MS/MS analyses.


  1. D. M. T. Loo, L. Monnens, T. van der Velden, M. Karmali, L. van de Heuvel, and V. van Hinsbergh, “Shiga toxin-1 affects nitric oxide production by human glomerular endothelial and mesangial cells,” Pediatric Nephrology, vol. 21, no. 12, pp. 1815–1823, 2006. View at: Publisher Site | Google Scholar
  2. S. Razzaq, “Hemolytic uremic syndrome: an emerging health risk,” The American Family Physician, vol. 74, no. 6, pp. 991–996, 2006. View at: Google Scholar
  3. S. Loos, T. Ahlenstiel, B. Kranz et al., “An outbreak of Shiga toxin-producing Escherichia coli O104:H4 hemolytic uremic syndrome in Germany: presentation and short-term outcome in children,” Clinical Infectious Diseases, vol. 55, no. 6, pp. 753–759, 2012. View at: Publisher Site | Google Scholar
  4. A. Gerber, H. Karch, F. Allerberger, H. M. Verweyen, and L. B. Zimmerhackl, “Clinical course and the role of Shiga toxin-producing Escherichia coli infection in the hemolytic-uremic syndrome in pediatric patients, 1997–2000, in Germany and Austria: a prospective study,” Journal of Infectious Diseases, vol. 186, no. 4, pp. 493–500, 2002. View at: Publisher Site | Google Scholar
  5. S. E. Richardson, M. A. Karmali, L. E. Becker, and C. R. Smith, “The histopathology of the hemolytic uremic syndrome associated with verocytotoxin-producing Escherichia coli infections,” Human Pathology, vol. 19, no. 9, pp. 1102–1108, 1988. View at: Google Scholar
  6. C. D. Inward, A. J. Howie, M. M. Fitzpatrick, F. Rafaat, D. V. Milford, and C. M. Taylor, “Renal histopathology in fatal cases of diarrhoea-associated haemolytic uraemic syndrome,” Pediatric Nephrology, vol. 11, no. 5, pp. 556–559, 1997. View at: Publisher Site | Google Scholar
  7. N. Gordjani, A. H. Sutor, L. B. Zimmerhackl, and M. Brandis, “Hemolytic uremic syndromes in childhood,” Seminars in Thrombosis and Hemostasis, vol. 23, no. 3, pp. 281–293, 1997. View at: Google Scholar
  8. A. X. Garg, R. S. Suri, N. Barrowman et al., “Long-term renal prognosis of diarrhea-associated hemolytic uremic syndrome: a systematic review, meta-analysis, and meta-regression,” The Journal of the American Medical Association, vol. 290, no. 10, pp. 1360–1370, 2003. View at: Publisher Site | Google Scholar
  9. C. A. Lingwood, “Shiga toxin receptor glycolipid binding. Pathology and utility,” Methods in Molecular Medicine, vol. 73, pp. 165–186, 2003. View at: Google Scholar
  10. D. D. Rees, R. M. J. Palmer, and S. Moncada, “Role of endothelium-derived nitric oxide in the regulation of blood pressure,” Proceedings of the National Academy of Sciences of the United States of America, vol. 86, no. 9, pp. 3375–3378, 1989. View at: Google Scholar
  11. J. P. Cooke and V. J. Dzau, “Nitric oxide synthase: role in the genesis of vascular disease,” Annual Review of Medicine, vol. 48, pp. 489–509, 1997. View at: Publisher Site | Google Scholar
  12. L. J. Ignarro, “Biosynthesis and metabolism of endothelium-derived nitric oxide,” Annual Review of Pharmacology and Toxicology, vol. 30, pp. 535–560, 1990. View at: Google Scholar
  13. D. Tsikas, F. Gutzki, and D. O. Stichtenoth, “Circulating and excretory nitrite and nitrate as indicators of nitric oxide synthesis in humans: methods of analysis,” European Journal of Clinical Pharmacology, vol. 62, no. 13, pp. 51–59, 2006. View at: Publisher Site | Google Scholar
  14. M. A. Marletta, “Nitric oxide synthase structure and mechanism,” The Journal of Biological Chemistry, vol. 268, no. 17, pp. 12231–12234, 1993. View at: Google Scholar
  15. V. Achan, M. Broadhead, M. Malaki et al., “Asymmetric dimethylarginine causes hypertension and cardiac dysfunction in humans and is actively metabolized by dimethylarginine dimethylaminohydrolase,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 23, no. 8, pp. 1455–1459, 2003. View at: Publisher Site | Google Scholar
  16. J. M. Leiper and P. Vallance, “The synthesis and metabolism of asymmetric dimethylarginine (ADMA),” European Journal of Clinical Pharmacology, vol. 62, no. 13, pp. 33–38, 2006. View at: Publisher Site | Google Scholar
  17. J. T. Kielstein, S. M. Bode-Böger, J. C. Frölich, H. Haller, and R. H. Böger, “Relationship of asymmetric dimethylarginine to dialysis treatment and atherosclerotic disease,” Kidney International Supplements, vol. 78, pp. S9–S13, 2001. View at: Publisher Site | Google Scholar
  18. H. Herlitz, A. Petersson, L. Sigström, A. Wennmalm, and G. Westberg, “The arginine-nitric oxide pathway in thrombotic microangiopathy,” Scandinavian Journal of Urology and Nephrology, vol. 31, no. 5, pp. 477–479, 1997. View at: Google Scholar
  19. T. Lücke, N. Kanzelmeyer, M. J. Kemper, D. Tsikas, and A. M. Das, “Developmental changes in the L-arginine/nitric oxide pathway from infancy to adulthood: plasma asymmetric dimethylarginine levels decrease with age,” Clinical Chemistry and Laboratory Medicine, vol. 45, no. 11, pp. 1525–1530, 2007. View at: Publisher Site | Google Scholar
  20. T. Lücke, N. Kanzelmeyer, K. Chobanyan et al., “Elevated asymmetric dimethylarginine (ADMA) and inverse correlation between circulating ADMA and glomerular filtration rate in children with sporadic focal segmental glomerulosclerosis (FSGS),” Nephrology Dialysis Transplantation, vol. 23, no. 2, pp. 734–740, 2008. View at: Publisher Site | Google Scholar
  21. G. Facorro, F. Aguirre, L. Florentin et al., “Oxidative stress and membrane fluidity in erythrocytes from patients with hemolytic uremic syndrome,” Acta Physiologica, Pharmacologica et Therapeutica Latinoamericana, vol. 47, no. 3, pp. 137–146, 1997. View at: Google Scholar
  22. V. Ferraris, A. Acquier, J. R. Ferraris, G. Vallejo, C. Paz, and C. F. Mendez, “Oxidative stress status during the acute phase of haemolytic uraemic syndrome,” Nephrology Dialysis Transplantation, vol. 26, no. 3, pp. 858–864, 2011. View at: Publisher Site | Google Scholar
  23. S. A. Gomez, M. J. Abrey-Recalde, C. A. Panek et al., “The oxidative stress induced in vivo by Shiga toxin-2 contributes to the pathogenicity of haemolytic uraemic syndrome,” Clinical and Experimental Immunology, vol. 173, no. 3, pp. 463–472, 2013. View at: Publisher Site | Google Scholar
  24. T. Kajiume, A. Nagita, S. Yoshimi, K. Kobayashi, and N. Kataoka, “A case of hemolytic uremic syndrome improved with nitric oxide,” Bone Marrow Transplantation, vol. 25, no. 1, pp. 109–110, 2000. View at: Google Scholar
  25. V. V. Pham, D. O. Stichtenoth, and D. Tsikas, “Nitrite correlates with 3-nitrotyrosine but not with the F2-isoprostane 15(S)-8-iso-PGF2α in urine of rheumatic patients,” Nitric Oxide—Biology and Chemistry, vol. 21, no. 3-4, pp. 210–215, 2009. View at: Publisher Site | Google Scholar
  26. D. Tsikas, B. Schubert, F. Gutzki, J. Sandmann, and J. C. Frölich, “Quantitative determination of circulating and urinary asymmetric dimethylarginine (ADMA) in humans by gas chromatography-tandem mass spectrometry as methyl ester tri(N-pentafluoropropionyl) derivative,” Journal of Chromatography B, vol. 798, no. 1, pp. 87–99, 2003. View at: Publisher Site | Google Scholar
  27. D. Tsikas, “Simultaneous derivatization and quantification of the nitric oxide metabolites nitrite and nitrate in biological fluids by gas chromatography/mass spectrometry,” Analytical Chemistry, vol. 72, no. 17, pp. 4064–4072, 2000. View at: Google Scholar
  28. D. Tsikas, A. Wolf, A. Mitschke, F. Gutzki, W. Will, and M. Bader, “GC-MS determination of creatinine in human biological fluids as pentafluorobenzyl derivative in clinical studies and biomonitoring: inter-laboratory comparison in urine with Jaffé, HPLC and enzymatic assays,” Journal of Chromatography B, vol. 878, no. 27, pp. 2582–2592, 2010. View at: Publisher Site | Google Scholar
  29. D. Tsikas, T. Thum, T. Becker et al., “Accurate quantification of dimethylamine (DMA) in human urine by gas chromatography-mass spectrometry as pentafluorobenzamide derivative: evaluation of the relationship between DMA and its precursor asymmetric dimethylarginine (ADMA) in health and disease,” Journal of Chromatography B, vol. 851, no. 1-2, pp. 229–239, 2007. View at: Publisher Site | Google Scholar
  30. A. Woywodt, A. D. Blann, T. Kirsch et al., “Isolation and enumeration of circulating endothelial cells by immunomagnetic isolation: proposal of a definition and a consensus protocol,” Journal of Thrombosis and Haemostasis, vol. 4, no. 3, pp. 671–677, 2006. View at: Publisher Site | Google Scholar
  31. J. Jacobi and P. S. Tsao, “Asymmetrical dimethylarginine in renal disease: limits of variation or variation limits? A systematic review,” The American Journal of Nephrology, vol. 28, no. 2, pp. 224–237, 2008. View at: Publisher Site | Google Scholar
  32. C. Bogdan, M. Röllinhoff, and A. Difenbach, “The role of nitric oxide in innate immunity,” Immunological Reviews, vol. 173, no. 1, pp. 17–26, 2000. View at: Publisher Site | Google Scholar
  33. T. Lücke, D. Tsikas, N. K. Kanzelmeyer et al., “Vaso-occlusion in Schimke-immuno-osseous dysplasia: is the NO pathway involved?” Hormone and Metabolic Research, vol. 38, no. 10, pp. 678–682, 2006. View at: Publisher Site | Google Scholar
  34. G. I. Dran, G. C. Fernández, C. J. Rubel et al., “Protective role of nitric oxide in mice with Shiga toxin-induced hemolytic uremic syndrome,” Kidney International, vol. 62, no. 4, pp. 1338–1348, 2002. View at: Publisher Site | Google Scholar
  35. J. Shao, T. Miyata, K. Yamada et al., “Protective role of nitric oxide in a model of thrombotic microangiopathy in rats,” Journal of the American Society of Nephrology, vol. 12, no. 10, pp. 2088–2097, 2001. View at: Google Scholar
  36. S. Tsuji, A. Iharada, T. Kimata, T. Shimo, M. Hirabayashi, and K. Kaneko, “Production of nitric oxide is lower in Shiga toxin-stimulated neutrophils of infants compared to those of children or adults,” Tohoku Journal of Experimental Medicine, vol. 228, no. 3, pp. 247–252, 2012. View at: Publisher Site | Google Scholar
  37. A. Wennmalm, G. Benthin, and A. Peterssen, “Dependence of the metabolism of nitric oxide (NO) in healthy human whole blood on the oxygenation of its red cell haemoglobin,” The British Journal of Pharmacology, vol. 106, no. 3, pp. 507–508, 1992. View at: Google Scholar
  38. C. D. Reiter, X. Wang, J. E. Tanus-Santos et al., “Cell-free hemoglobin limits nitric oxide bioavailability in sickle-cell disease,” Nature Medicine, vol. 8, no. 12, pp. 1383–1389, 2002. View at: Publisher Site | Google Scholar
  39. K. Cosby, K. S. Partovi, J. H. Crawford et al., “Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation,” Nature Medicine, vol. 9, no. 12, pp. 1498–1505, 2003. View at: Publisher Site | Google Scholar
  40. J. R. Pawloski, D. T. Hess, and J. S. Stamler, “Impaired vasodilation by red blood cells in sickle cell disease,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 7, pp. 2531–2536, 2005. View at: Publisher Site | Google Scholar
  41. I. Akinsheye and E. S. Klings, “Sickle cell anemia and vascular dysfunction: the nitric oxide connection,” Journal of Cellular Physiology, vol. 224, no. 3, pp. 620–625, 2010. View at: Publisher Site | Google Scholar
  42. J. H. Maley, G. F. Lasker, and P. J. Kadowitz, “Nitric oxide and disorders of the erythrocyte: emerging roles and therapeutic targets,” Cardiovascular and Hematological Disorders—Drug Targets, vol. 10, no. 4, pp. 284–291, 2010. View at: Google Scholar
  43. U. Dreißigacker, M. Suchy, N. Maassen, and D. Tsikas, “Human plasma concentrations of malondialdehyde (MDA) and the F2-isoprostane 15(S)-8-iso-PGF2α may be markedly compromised by hemolysis: evidence by GC-MS/MS and potential analytical and biological ramifications,” Clinical Biochemistry, vol. 43, no. 1-2, pp. 159–167, 2010. View at: Publisher Site | Google Scholar
  44. N. J. Parks, K. J. Krohn, C. A. Mathis et al., “Nitrogen-13-labeled nitrite and nitrate: distribution and metabolism after intratracheal administration,” Science, vol. 212, no. 4490, pp. 58–60, 1981. View at: Google Scholar
  45. D. Tsikas, K. Sutmöller, M. Maassen et al., “Even and carbon dioxide independent distribution of nitrite between plasma and erythrocytes of healthy humans at rest,” Nitric Oxide, vol. 31, pp. 31–37, 2013. View at: Publisher Site | Google Scholar
  46. R. Keimer, F. K. Stutzer, D. Tsikas, R. Troost, F. Gutzki, and J. C. Frölich, “Lack of oxidative stress during sustained therapy with isosorbide dinitrate and pentaerythrityl tetranitrate in healthy humans: a randomized, double-blind crossover study,” Journal of Cardiovascular Pharmacology, vol. 41, no. 2, pp. 284–292, 2003. View at: Publisher Site | Google Scholar
  47. D. Tsikas and M. W. Duncan, “Mass spectrometry and 3-nitrotyrosine: strategies, controversies, and our current perspective,” Mass Spectrometry Reviews, 2013. View at: Publisher Site | Google Scholar
  48. D. Tsikas, A. Böhmer, H. Großkopf et al., “Clinical-chemistry laboratory relevant hemolysis is unlikely to compromise human plasma concentration of free asymmetric dimethylarginine (ADMA),” Clinical Biochemistry, vol. 45, no. 16-17, pp. 1536–1538, 2012. View at: Publisher Site | Google Scholar
  49. C. Wolf, J. M. Lorenzen, S. Stein et al., “Urinary asymmetric dimethylarginine (ADMA) is a predictor of mortality risk in patients with coronary artery disease,” International Journal of Cardiology, vol. 156, no. 3, pp. 289–294, 2012. View at: Publisher Site | Google Scholar
  50. D. Giustarini, A. Milzani, I. Dalle-Donne, D. Tsikas, and R. Rossi, “N-Acetylcysteine ethyl ester (NACET): a novel lipophilic cell-permeable cysteine derivative with an unusual pharmacokinetic feature and remarkable antioxidant potential,” Biochemical Pharmacology, vol. 84, no. 11, pp. 1522–1533, 2012. View at: Publisher Site | Google Scholar

Copyright © 2014 Nele Kirsten Kanzelmeyer et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1110 Views | 473 Downloads | 3 Citations
 PDF  Download Citation  Citation
 Download other formatsMore
 Order printed copiesOrder

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.