Resveratrol Counteracts Inflammation in Human M1 and M2 Macrophages upon Challenge with 7-Oxo-Cholesterol: Potential Therapeutic Implications in Atherosclerosis
Figure 2
Analysis of macrophage endocytosis. Resveratrol prevented the impairment of endocytosis in M2 macrophages in response to 7-oxo-cholesterol (7oxo-C). M1 (a) and M2 (b) macrophages—pretreated or not with resveratrol (30 μM) for 1 hour and then incubated with 7oxo-C (15 μM) for 20 h or left unstimulated—were added with FITC-dextran (1 mg/mL) and incubated for 30 minutes at 37°C at 5% CO2. The cellular uptake was analyzed by flow cytometry. The results of one representative experiment of three are shown. The number in the histograms shows the mean fluorescence intensity.
