Research Article

Enhancement of Cellular Antioxidant-Defence Preserves Diastolic Dysfunction via Regulation of Both Diastolic Zn2+ and Ca2+ and Prevention of RyR2-Leak in Hyperglycemic Cardiomyocytes

Figure 5

Altered intracellular global either Ca2+ or Zn2+ changes in cardiomyocytes are normalized with either N-acetyl cysteine (NAC)-treatment of diabetic rats or NAC-incubation in diabetic cardiomyocytes. (a) Inset: representative Ca2+ or Zn2+ transients in freshly isolated cardiomyocytes loaded with either FluoZin-3 (low) or Fluo-3 (up) and field-stimulated at 0.2 Hz (left). The peak amplitude of the fluorescences related to either global Ca2+ or Zn2+ (the transient changes) is given as . The effect of NAC treatment of diabetic rats (DM + NAC group: 150 mg/kg, daily and intragastrically, for 4 weeks) or NAC incubation of diabetic cardiomyocytes (+NAC group; 1 mM, for 1 h at 37°C) on the time to peak fluorescence, TP (left), and half-decay time, DT50 (right), of fluorescences either FluoZin-3 (b) or Fluo-3 (c). Bars represent mean ± SEM for controls (CON; , ) and diabetics (DM; , ) as well as DM + NAC ( , ) and +NAC ( , ) groups, respectively. Significant at * versus CON.
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