BMP-2 Overexpression Augments Vascular Smooth Muscle Cell Motility by Upregulating Myosin Va via Erk Signaling
Myosin Va expression regulated by BMP-2. (a) qRT-PCR analysis revealed mRNA upregulation of the myosin Va gene associated with adenoviral-mediated BMP-2 overexpression in rat VSMCs. (b) Myosin Va expression reflects a dose-dependent response to BMP-2 administration. (c) Immunofluorescent assay characterizes actin and myosin Va expression. Myosin Va proteins were abundantly distributed in the cytoplasm of cells binding closed actin in BMP-2 overexpressing VSMCs, compared to control (which manifested only weakly positive signals).