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Oxidative Medicine and Cellular Longevity
Volume 2014 (2014), Article ID 427560, 14 pages
Research Article

A Novel Contrast-Induced Acute Kidney Injury Model Based on the 5/6-Nephrectomy Rat and Nephrotoxicological Evaluation of Iohexol and Iodixanol In Vivo

1Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai 200032, China
2Division of Nephrology, The Affiliated Changzhou No. 2 Hospital of Nanjing Medical College, Changzhou, Jiangsu 213003, China
3Shanghai Institute of Kidney and Dialysis, Shanghai 200032, China

Received 31 July 2014; Revised 6 October 2014; Accepted 14 October 2014; Published 11 November 2014

Academic Editor: Sidhartha D. Ray

Copyright © 2014 Tong-qiang Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Contrast-induced acute kidney injury (CI-AKI) is a serious complication in patients after administration of iodinated contrast media. Proper animal models of CI-AKI can help understand the mechanisms involved and prevent the disorder. We used the 5/6-nephrectomized (NE) rat to develop a CI-AKI model and to evaluate differences in the toxic effects on the kidney between iohexol and iodixanol. We found that six weeks after ablative surgery was the preferred time to induce CI-AKI. We compared multiple pretreatment plans and found that dehydration for 48 hours before iodixanol (320, 10 mL/kg) administration was optimal to induce CI-AKI in the 5/6 NE rats. Compared with iodixanol, iohexol induced a significantly greater reduction in renal function, severe renal tissue damage, intrarenal hypoxia, and apoptotic tubular cells. Iohexol and iodixanol resulted in similarly marked increases in levels of inflammation and oxidative stress. In summary, the 5/6 NE rat combined with dehydration for 48 hours is a useful pretreatment to establish a novel and reliable CI-AKI model. Iohexol induced more severe CI-AKI than iodixanol in this model.