Exercise Training Preserves Ischemic Preconditioning in Aged Rat Hearts by Restoring the Myocardial Polyamine Pool

Figure 5

(a) The results from six similar experiments of mitochondrial swelling are shown. Mitochondria (0.5 mg/mL) were incubated in standard incubation medium. After 1.5 min of incubation, the mitochondria were loaded with 200 μM CaCl₂. Data are expressed as the mean ± SE. versus the control group; ^† versus the OPC group; versus the Ex-OPC group. (b) Changes in the mitochondrial expression of Cyt c, HSP70, and COX-2 as determined by western blot. A quantitative analysis of the mitochondrial Cyt c (c), HSP70 (d), and COX-2 (e) expression levels is also shown. VDAC was used as an internal control. Data are presented as the mean ± SE ( per group). versus the OC group; ^† versus the OPC group; versus the Ex-OPC group. (f and g) Dose-dependent effect of spermine and spermidine on the state 3 respiratory rate in cardiac mitochondria isolated from young hearts in the presence of 100 μM H₂O₂ (-7) for 10 min at 30°C. versus basal conditions; ^†, ^†† versus H₂O₂ treatment.

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