Effects of Treating Old Rats with an Aqueous Agaricus blazei Extract on Oxidative and Functional Parameters of the Brain Tissue and Brain Mitochondria

Table 3

Effects of the A. blazei extract treatment on the oxidation of NADH, succinate, and TMPD-ascorbate by disrupted brain mitochondria of old rats. The NADH oxidase, succinate oxidase, and oxidation of ascorbate mediated by TMPD were measured polarographically using freeze-thawing disrupted mitochondria as described in Materials and Methods. The values represent the mean ± standard errors of the mean.

Enzymatic activities	Experimental groups
	(1) Young rats	(2) Old rats	(3) A. blazei-treated old rats
nmol O2 min^−1 mg protein^−1
NADH oxidase	58.56±2.19 (𝑛=6)	46.12±4.84^# (𝑛=6)	46.44±4.77^∗∗ (𝑛=7)
Succinate oxidase	38.12±1.47 (𝑛=6)	31.50±1.53^# (𝑛=5)	32.27±1.32 (𝑛=5)
TMPD-ascorbate oxidation	113.89±5.75 (𝑛=4)	79.40±3.03^# (𝑛=5)	98.66±6.25 (𝑛=5)

^#𝑃<0.05 for (2) versus (1) (ANOVA and Student-Newman-Keuls post hoc testing).
^∗∗𝑃<0.05 for (3) versus (1) ANOVA and Student-Newman-Keuls post hoc testing).