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Oxidative Medicine and Cellular Longevity
Volume 2014, Article ID 617351, 12 pages
Research Article

Impaired 8-Hydroxyguanine Repair Activity of MUTYH Variant p.Arg109Trp Found in a Japanese Patient with Early-Onset Colorectal Cancer

1Department of Tumor Pathology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi Ward, Hamamatsu, Shizuoka 431-3192, Japan
2Division of Cancer Development System, National Cancer Center Research Institute, Tokyo 104-0045, Japan
3Research Center for Environmental Quality Management, Kyoto University, Shiga 520-0811, Japan
4Department of Preventive Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan

Received 29 November 2013; Revised 5 February 2014; Accepted 11 February 2014; Published 23 March 2014

Academic Editor: Antonio Ayala

Copyright © 2014 Kazuya Shinmura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

We provided the following Supplementary Tables S1-S5: Supplementary Table S1 (Primers used for PCR amplification of the MUTYH gene), Supplementary Table S2 (Primers used for PCR amplification of the OGG1 gene), Supplementary Table S3 (Primers used for PCR-CTPP of the MUTYH c.55C>T and c.325C>T variants), Supplementary Table S4 (MUTYH nucleotide variations in its coding region and splice-site region found in 34 Japanese patients with early-onset CRC), and Supplementary Table S5 (OGG1 nucleotide variations in its coding region found in 34 Japanese patients with early-onset CRC).

We also provided the following Supplementary Figures S1 and S2: Supplementary Figure S1 [Identification of novel OGG1 variants of c.949-89G>T and c.966C>T (p.Asp322Asp) in Japanese patients with early-onset CRC by sequencing analysis] and Supplementary Figure S2 [The distribution of supF base substitution-type mutations in a supF forward mutation assay using the pMY189 plasmid containing 8-hydroxyguanine (8OHG) at position 159 of supF in H1299 human cell lines inducibly expressing MUTYH protein].

  1. Supplementary Materials